Journal: Journal of neurochemistry

Article Title: Altered MAP kinase phosphorylation and impaired motor coordination in PTPRR deficient mice.

doi: 10.1111/j.1471-4159.2006.04398.x

Figure Lengend Snippet: Fig. 3 Altered MAPK phosphorylation levels in Ptprr)/) mouse brain. Degree of ERK1/2 (a), p38 (b) and JNK1/2 (c) biphosphorylation in cerebellum and cerebrum of wildtype (+/+, white bars) and Ptprr)/) ()/ ), black bars) mice as determined by Western analyses of tissue lysates from individual mice (n = 6 for each tissue and genotype group) using phospho-specific antibodies. Representative examples (three out of the six for each tissue and genotype group) of phos- phorylated ERK1/p44 and ERK2/p42 (pERK) and total ERK1/2 (tot- ERK) immunofluorescent signals are depicted above the corresponding bars. Phosphorylated ERK1/2, p38 and JNK1/2 signals were normalized to total ERK1/2, p38 and JNK1/2 levels, respectively, and plotted as relative phosporylation levels. Results are the mean ± SEM of six samples per group and are representative of at least three independent experiments (*p < 0.0001, **p < 0.01).

Article Snippet: Polyclonal antisera against total ERK1 (1 : 8000), p38 (1 : 2000), and JNK1 (1 : 500) were obtained from Santa Cruz Biotechnology (sc-93, sc-535 and sc-474, respectively; Santa Cruz, CA, USA), antiserum against total MEK1 (1 : 1000) was from Biosource (AH01102), monoclonal antibody ERK-PT115 (1 : 500) against threonine-phosphorylated ERK1/2 was from Sigma-Aldrich (St Louis, MO, USA) (M7802), antiserum against Elk-1 (1 : 1000) and monoclonal phospho-specific antibodies against ERK1/2 (1 : 2000), p38 (1 : 2000), JNK1 (1 : 500), MEK1/2 (1 : 1000), Elk-1 (1 : 1000) and S6 ribosomal protein (1 : 500) were from Cell Signalling Technology (Beverly, MA, USA) (nos 9182, 9106, 9216, 9255, 9121, 9186 and 4856, respectively).

Techniques: Phospho-proteomics, Western Blot

Journal: Molecular human reproduction

Article Title: Ectopic endometrial cells express high concentrations of interleukin (IL)-8 in vivo regardless of the menstrual cycle phase and respond to oestradiol by up-regulating IL-1-induced IL-8 expression in vitro.

doi: 10.1093/molehr/7.9.859

Figure Lengend Snippet: Figure 1. Localization of interleukin-8 (IL-8) in endometriotic lesions by immunohistochemistry. Sections were incubated with polyclonal rabbit anti-IL-8 antibody, then with biotin-conjugated anti-rabbit antibody and streptavidin–peroxidase. The immunoreaction was revealed with DAB as chromogen, and haematoxylin which stains the nucleus in blue was used for counterstaining. Note the intense brown positive immunostaining in endometriotic glands and stroma from (A) an ovarian endometriotic lesion, stage IV, proliferative phase, day 4 (patient no. 6, Table I), and (B) the inner lining of ovarian endometrioma, stage IV, secretory phase, day 22 (patient no. 10, Table I). (C and D) control sections incubated with normal rabbit IgG instead of the primary polyclonal rabbit antibody showing no immunostaining. Scale bar 50 µm.

Article Snippet: Immunostaining was performed using a polyclonal rabbit anti-IL-8 antibody [10 μg/ml in PBS containing 1% bovine serum albumin (BSA)] (Biosource International, Camarillo, CA, USA), a biotin-conjugated affinity-purified goat anti-rabbit antibody (H L) (2 μg/ml) (Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA), peroxidase-conjugated streptavidin (1.3 μg/ml) (Jackson ImmunoResearch Laboratories), diaminobenzidine (Sigma Chemical Co, St Louis, MO, USA) as chromogen and haematoxylin for counterstaining.

Techniques: Immunohistochemistry, Incubation, Immunostaining, Control